› 4mfh

X-ray diffraction
1.54Å resolution

Crystal Structure of M121G Azurin

Released:
DOI: 10.2210/pdb4mfh/pdb
PDB entry 4mfh coloured by chain, front view.
PDB entry 4mfh coloured by chain, side view.
PDB entry 4mfh coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Copper-sulfenate complex from oxidation of a cavity mutant of Pseudomonas aeruginosa azurin.
Sieracki NA, Tian S, Hadt RG, Zhang JL, Woertink JS, Nilges MJ, Sun F, Solomon EI, Lu Y
Proc Natl Acad Sci U S A 111 924-9 (2014)
PMID: 24390543

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 6 x CU
Ligand TRS 3 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 55.747Å b: 145.781Å c: 97.092Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design and engineering of artificial oxygen-activating metalloenzymes.
Nastri et al. (2016)
6 other articles

2 mentions without citation

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
1 more