› 4me3

X-ray diffraction
1.79Å resolution

1.8 Angstrom Crystal Structure of the N-terminal Domain of an Archaeal MCM

Released:
DOI: 10.2210/pdb4me3/pdb
PDB entry 4me3 coloured by chain, front view.
PDB entry 4me3 coloured by chain, side view.
PDB entry 4me3 coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Primary publication:
The 1.8-Å crystal structure of the N-terminal domain of an archaeal MCM as a right-handed filament.
Fu Y, Slaymaker IM, Wang J, Wang G, Chen XS
J Mol Biol 426 1512-23 (2014)
PMID: 24378617

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 31.45 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HK10 (Residues: 1-262; Coverage: 38%)
Gene name: Ta0799
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1, APS BEAMLINE 23-ID-D
Spacegroup: P61
Unit cell:
a: 93.46Å b: 93.46Å c: 56.231Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanisms and regulation of DNA replication initiation in eukaryotes.
Parker et al. (2017)
2 more

7 mentions without citation

Unwinding 20 Years of the Archaeal Minichromosome Maintenance Helicase.
Kelman et al. (2020)
6 more