› 4mab

X-ray diffraction
1.9Å resolution

Resolving Cys to Ala variant of Salmonella Alkyl Hydroperoxide Reductase C in its substrate-ready conformation

Released:
DOI: 10.2210/pdb4mab/pdb
PDB entry 4mab coloured by chain, front view.
PDB entry 4mab coloured by chain, side view.
PDB entry 4mab coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
The sensitive balance between the fully folded and locally unfolded conformations of a model peroxiredoxin.
Perkins A, Nelson KJ, Williams JR, Parsonage D, Poole LB, Karplus PA
Biochemistry 52 8708-21 (2013)
PMID: 24175952

Function and Biology Details

Reaction catalysed: 
NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase C Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 186 amino acids
Theoretical weight: 20.61 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A251 (Residues: 2-187; Coverage: 100%)
Gene names: STM0608, ahpC
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand K 3 x K
Ligand GOL 4 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 127.23Å b: 172.42Å c: 136.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling.
Perkins et al. (2015)
6 more

2 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
1 more