› 4m56

X-ray diffraction
2.3Å resolution

The Structure of Wild-type MalL from Bacillus subtilis

Released:
DOI: 10.2210/pdb4m56/pdb
PDB entry 4m56 coloured by chain, front view.
PDB entry 4m56 coloured by chain, side view.
PDB entry 4m56 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates.
Hobbs JK, Jiao W, Easter AD, Parker EJ, Schipper LA, Arcus VL
ACS Chem Biol 8 2388-93 (2013)
PMID: 24015933

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oligo-1,6-glucosidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 561 amino acids
Theoretical weight: 66.16 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O06994 (Residues: 1-561; Coverage: 100%)
Gene names: BSU34560, malL, yvdL
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLO 2 x GLO
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 61.65Å b: 100.26Å c: 101.49Å
α: 90° β: 103.28° γ: 90°
R-values:
R R work R free
0.19 0.187 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Enzymatic Transition States and Drug Design.
Schramm VL. (2018)
4 more

5 mentions without citation

Dynamical origins of heat capacity changes in enzyme-catalysed reactions.
van der Kamp et al. (2018)
4 more