› 4lwz

X-ray diffraction
2.55Å resolution

Crystal structure of Myo5b globular tail domain in complex with inactive Rab11a

Released:
DOI: 10.2210/pdb4lwz/pdb
PDB entry 4lwz coloured by chain, front view.
PDB entry 4lwz coloured by chain, side view.
PDB entry 4lwz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of myosin V Rab GTPase-dependent cargo recognition.
Pylypenko O, Attanda W, Gauquelin C, Lahmani M, Coulibaly D, Baron B, Hoos S, Titus MA, England P, Houdusse AM
Proc Natl Acad Sci U S A 110 20443-8 (2013)
PMID: 24248336

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158627 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-11A Chains: A, C
Molecule details ›
Chains: A, C
Length: 177 amino acids
Theoretical weight: 19.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62491 (Residues: 1-177; Coverage: 82%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Unconventional myosin-Vb Chains: B, D
Molecule details ›
Chains: B, D
Length: 427 amino acids
Theoretical weight: 49.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULV0 (Residues: 1456-1848; Coverage: 21%)
Gene names: KIAA1119, MYO5B
Sequence domains: DIL domain

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 67.68Å b: 143.14Å c: 162.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.295
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Orchestration of cell surface proteins by Rab11.
Welz et al. (2014)
15 more

2 mentions without citation

Structural basis of myosin V Rab GTPase-dependent cargo recognition.
Pylypenko et al. (2013)
1 more