› 4lt0

X-ray diffraction
2.1Å resolution

HEWL co-crystallized with Carboplatin in non-NaCl conditions: crystal 1 processed using the EVAL software package

Released:
DOI: 10.2210/pdb4lt0/pdb
PDB entry 4lt0 coloured by chain, front view.
PDB entry 4lt0 coloured by chain, side view.
PDB entry 4lt0 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Carboplatin binding to histidine.
Tanley SW, Diederichs K, Kroon-Batenburg LM, Levy C, Schreurs AM, Helliwell JR
Acta Crystallogr F Struct Biol Commun 70 1135-42 (2014)
PMID: 25195881

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand DMS 4 x DMS
Ligand QPT 1 x QPT
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P43212
Unit cell:
a: 76.745Å b: 76.745Å c: 36.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.283

Quick links

Citations

14 citation in other articles

Cisplatin binding to human serum albumin: a structural study.
Ferraro et al. (2015)
13 more

2 mentions without citation

Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin et al. (2015)
1 more