› 4loq

X-ray diffraction
2.32Å resolution

Structural basis of autoactivation of p38 alpha induced by TAB1 (Tetragonal crystal form with bound sulphate)

Released:
DOI: 10.2210/pdb4loq/pdb
PDB entry 4loq coloured by chain, front view.
PDB entry 4loq coloured by chain, side view.
PDB entry 4loq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mechanism and consequence of the autoactivation of p38α mitogen-activated protein kinase promoted by TAB1.
DeNicola GF, Martin ED, Chaikuad A, Bassi R, Clark J, Martino L, Verma S, Sicard P, Tata R, Atkinson RA, Knapp S, Conte MR, Marber MS
Nat Struct Mol Biol 20 1182-90 (2013)
PMID: 24037507

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155655 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 14 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 41.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47811 (Residues: 1-360; Coverage: 100%)
Gene names: Crk1, Csbp1, Csbp2, Mapk14
Sequence domains: Protein kinase domain
Structure domains:
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 29 amino acids
Theoretical weight: 3.1 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q8CF89 (Residues: 384-412; Coverage: 6%)
Gene names: Map3k7ip1, Tab1

Ligands and Environments

3 bound ligands:
Ligand SB4 4 x SB4
Ligand SO4 8 x SO4
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41
Unit cell:
a: 87.1Å b: 87.1Å c: 228.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.192 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Diversity and versatility of p38 kinase signalling in health and disease.
Canovas et al. (2021)
16 more

3 mentions without citation

Mechanism and consequence of the autoactivation of p38α mitogen-activated protein kinase promoted by TAB1.
DeNicola et al. (2013)
2 more