› 4lmg

X-ray diffraction
2.2Å resolution

Crystal structure of AFT2 in complex with DNA

Released:
DOI: 10.2210/pdb4lmg/pdb
PDB entry 4lmg coloured by chain, front view.
PDB entry 4lmg coloured by chain, side view.
PDB entry 4lmg coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular mechanism and structure of the Saccharomyces cerevisiae iron regulator Aft2.
Poor CB, Wegner SV, Li H, Dlouhy AC, Schuermann JP, Sanishvili R, Hinshaw JR, Riggs-Gelasco PJ, Outten CE, He C
Proc Natl Acad Sci U S A 111 4043-8 (2014)
PMID: 24591629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117792 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Iron-regulated transcriptional activator AFT2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 18.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08957 (Residues: 38-193; Coverage: 38%)
Gene names: AFT2, YPL202C
Sequence domains: Transcription factor AFT
5'-D(*TP*AP*AP*TP*GP*GP*GP*TP*GP*CP*AP*CP*T)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
5'-D(*AP*AP*GP*TP*GP*CP*AP*CP*CP*CP*AP*TP*T)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 41.536Å b: 127.453Å c: 70.766Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.192 0.189 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Essential functions of iron-requiring proteins in DNA replication, repair and cell cycle control.
Zhang C. (2014)
18 more

1 mention without citation

Molecular mechanism and structure of the Saccharomyces cerevisiae iron regulator Aft2.
Poor et al. (2014)