› 4ljh

X-ray diffraction
1.45Å resolution

Crystal Structure of Pseudomonas aeruginosa Lectin LecA Complexed with 1-Methyl-3-indolyl-b-D-galactopyranoside at 1.45 A Resolution

Released:
DOI: 10.2210/pdb4ljh/pdb
PDB entry 4ljh coloured by chain, front view.
PDB entry 4ljh coloured by chain, side view.
PDB entry 4ljh coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
CH-π "T-shape" interaction with histidine explains binding of aromatic galactosides to Pseudomonas aeruginosa lectin LecA.
Kadam RU, Garg D, Schwartz J, Visini R, Sattler M, Stocker A, Darbre T, Reymond JL
ACS Chem Biol 8 1925-30 (2013)
PMID: 23869965

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA-I galactophilic lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05097 (Residues: 1-122; Coverage: 100%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand MHD 4 x MHD
Ligand GAL 4 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 49.6Å b: 68.2Å c: 159.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Pathoblockers or antivirulence drugs as a new option for the treatment of bacterial infections.
Calvert et al. (2018)
4 more

1 mention without citation

Photoswitchable precision glycooligomers and their lectin binding.
Ponader et al. (2014)