› 4lim

X-ray diffraction
1.63Å resolution

Crystal structure of the catalytic subunit of yeast primase

Released:
DOI: 10.2210/pdb4lim/pdb
PDB entry 4lim coloured by chain, front view.
PDB entry 4lim coloured by chain, side view.
PDB entry 4lim coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Insights into eukaryotic primer synthesis from structures of the p48 subunit of human DNA primase.
Vaithiyalingam S, Arnett DR, Aggarwal A, Eichman BF, Fanning E, Chazin WJ
J Mol Biol 426 558-69 (2014)
PMID: 24239947

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase small subunit Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 45.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P10363 (Residues: 8-396; Coverage: 95%)
Gene names: PRI1, YIB8C, YIR008C
Sequence domains: DNA primase small subunit
Structure domains: DNA primase, PRIM domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 37.166Å b: 43.624Å c: 69.383Å
α: 83.11° β: 83.25° γ: 78°
R-values:
R R work R free
0.156 0.155 0.189
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.
Guilliam et al. (2015)
3 more

2 mentions without citation

Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?
Boudet et al. (2015)
1 more