› 4lev

X-ray diffraction
1.95Å resolution

Structure of human cGAS

Released:
DOI: 10.2210/pdb4lev/pdb
PDB entry 4lev coloured by chain, front view.
PDB entry 4lev coloured by chain, side view.
PDB entry 4lev coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cyclic GMP-AMP synthase is activated by double-stranded DNA-induced oligomerization.
Li X, Shu C, Yi G, Chaton CT, Shelton CL, Diao J, Zuo X, Kao CC, Herr AB, Li P
Immunity 39 1019-31 (2013)
PMID: 24332030

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 43.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 212.981Å b: 47.715Å c: 86.869Å
α: 90° β: 113.89° γ: 90°
R-values:
R R work R free
0.169 0.167 0.202
Expression system: Escherichia coli

Quick links

Citations

130 review citations

Innate immune sensing and signaling of cytosolic nucleic acids.
Wu et al. (2014)
129 more

10 mentions without citation

Small molecules targeting the innate immune cGAS‒STING‒TBK1 signaling pathway.
Ding et al. (2020)
9 more