› 4lcu

X-ray diffraction
2.75Å resolution

Structure of KcsA with E118A mutation

Released:
DOI: 10.2210/pdb4lcu/pdb
PDB entry 4lcu coloured by chain, front view.
PDB entry 4lcu coloured by chain, side view.
PDB entry 4lcu coloured by chain, top view.
Source organisms:
Primary publication:
Molecular interactions involved in proton-dependent gating in KcsA potassium channels.
Posson DJ, Thompson AN, McCoy JG, Nimigean CM
J Gen Physiol 142 613-24 (2013)
PMID: 24218397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209478 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab light chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab heavy chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 131 amino acids
Theoretical weight: 14.11 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 2-124; Coverage: 77%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand K 6 x K
Ligand DGA 1 x DGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4
Unit cell:
a: 155.952Å b: 155.952Å c: 75.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.216
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

3 review citations

Channel function reconstitution and re-animation: a single-channel strategy in the postcrystal age.
Oiki S. (2015)
2 more

1 mention without citation

Molecular interactions involved in proton-dependent gating in KcsA potassium channels.
Posson et al. (2013)