› 4l73

X-ray diffraction
2.5Å resolution

Ca2+-bound MthK RCK domain at 2.5 Angstrom

Released:
DOI: 10.2210/pdb4l73/pdb
PDB entry 4l73 coloured by chain, front view.
PDB entry 4l73 coloured by chain, side view.
PDB entry 4l73 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain.
Smith FJ, Pau VP, Cingolani G, Rothberg BS
Nat Commun 4 2621 (2013)
PMID: 24126388

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.89 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 5 x CA
Ligand NA 8 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 67.96Å b: 67.96Å c: 159.891Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.226
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure of potassium channels.
Kuang et al. (2015)
1 more

2 mentions without citation

Understanding the conformational motions of RCK gating rings.
Giraldez et al. (2017)
1 more