› 4l5s

X-ray diffraction
2.94Å resolution

p202 HIN1 in complex with 12-mer dsDNA

Released:
DOI: 10.2210/pdb4l5s/pdb
PDB entry 4l5s coloured by chain, front view.
PDB entry 4l5s coloured by chain, side view.
PDB entry 4l5s coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Molecular mechanism for p202-mediated specific inhibition of AIM2 inflammasome activation.
Yin Q, Sester DP, Tian Y, Hsiao YS, Lu A, Cridland JA, Sagulenko V, Thygesen SJ, Choubey D, Hornung V, Walz T, Stacey KJ, Wu H
Cell Rep 4 327-39 (2013)
PMID: 23850291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero hexamer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-117777 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Interferon-activable protein 202 Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 22.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9R002 (Residues: 46-243; Coverage: 45%)
Gene names: Ifi202, Ifi202a, Ifi202b
Sequence domains: HIN-200/IF120x domain
Structure domains: Nucleic acid-binding proteins
12-mer DNA Chains: C, D, E, F, G, H, I
Molecule details ›
Chains: C, D, E, F, G, H, I
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 111.309Å b: 156.118Å c: 117.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

38 review citations

Inflammasomes: mechanism of assembly, regulation and signalling.
Broz et al. (2016)
37 more

4 mentions without citation

Activation and Immune Regulation Mechanisms of PYHIN Family During Microbial Infection.
Fan et al. (2021)
3 more