› 4l4g

X-ray diffraction
1.55Å resolution

Structure of cyanide and camphor bound P450cam mutant L358P/K178G

Released:
DOI: 10.2210/pdb4l4g/pdb
PDB entry 4l4g coloured by chain, front view.
PDB entry 4l4g coloured by chain, side view.
PDB entry 4l4g coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Synergistic effects of mutations in cytochrome P450cam designed to mimic CYP101D1.
Batabyal D, Li H, Poulos TL
Biochemistry 52 5396-402 (2013)
PMID: 23865948

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.6 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand CYN 1 x CYN
Ligand CAM 1 x CAM
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 57.072Å b: 58.79Å c: 57.072Å
α: 90° β: 105.02° γ: 90°
R-values:
R R work R free
0.174 0.172 0.212
Expression system: Escherichia coli

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Citations

2 review citations

Fluorinated porphyrinoids as efficient platforms for new photonic materials, sensors, and therapeutics.
Bhupathiraju et al. (2016)
1 more

1 mention without citation

Synergistic effects of mutations in cytochrome P450cam designed to mimic CYP101D1.
Batabyal et al. (2013)