› 4l13

X-ray diffraction
1.66Å resolution

Crystal structure of Ligand Free EGFP-based Calcium Sensor CatchER

Released:
DOI: 10.2210/pdb4l13/pdb
PDB entry 4l13 coloured by chain, front view.
PDB entry 4l13 coloured by chain, side view.
PDB entry 4l13 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.
Zhang Y, Reddish F, Tang S, Zhuo Y, Wang YF, Yang JJ, Weber IT
Acta Crystallogr D Biol Crystallogr 69 2309-19 (2013)
PMID: 24311573

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.14 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-230; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 61.401Å b: 88.534Å c: 118.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Calcium Dynamics Mediated by the Endoplasmic/Sarcoplasmic Reticulum and Related Diseases.
Reddish et al. (2017)
2 more

4 mentions without citation

Effect of Ca²⁺ on the steady-state and time-resolved emission properties of the genetically encoded fluorescent sensor CatchER.
Zhuo et al. (2015)
3 more