› 4kxy

X-ray diffraction
1.26Å resolution

Human transketolase in complex with ThDP analogue (R)-2-(1,2-dihydroxyethyl)-3-deaza-ThDP

Released:
DOI: 10.2210/pdb4kxy/pdb
PDB entry 4kxy coloured by chain, front view.
PDB entry 4kxy coloured by chain, side view.
PDB entry 4kxy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate.
Lüdtke S, Neumann P, Erixon KM, Leeper F, Kluger R, Ficner R, Tittmann K
Nat Chem 5 762-7 (2013)
PMID: 23965678

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 637 amino acids
Theoretical weight: 69.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29401 (Residues: 1-623; Coverage: 100%)
Gene name: TKT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand 1U0 2 x 1U0
4 bound ligands:
Ligand EDO 27 x EDO
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 73.15Å b: 86.05Å c: 92.73Å
α: 90° β: 94.12° γ: 90°
R-values:
R R work R free
0.165 0.154 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Transition metal catalysis in confined spaces.
Leenders et al. (2015)
4 more