› 4kvs

X-ray diffraction
1.67Å resolution

Crystal Structure of Prochlorococcus marinus aldehyde-deformylating oxygenase (mutant A134F)

Released:
DOI: 10.2210/pdb4kvs/pdb
PDB entry 4kvs coloured by chain, front view.
PDB entry 4kvs coloured by chain, side view.
PDB entry 4kvs coloured by chain, top view.
Source organism: Prochlorococcus marinus str. MIT 9313
Primary publication:
Production of propane and other short-chain alkanes by structure-based engineering of ligand specificity in aldehyde-deformylating oxygenase.
Khara B, Menon N, Levy C, Mansell D, Das D, Marsh EN, Leys D, Scrutton NS
Chembiochem 14 1204-8 (2013)
PMID: 23757044

Function and Biology Details

Reaction catalysed: 
A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde decarbonylase Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.34 KDa
Source organism: Prochlorococcus marinus str. MIT 9313
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7V6D4 (Residues: 1-243; Coverage: 100%)
Gene name: PMT_1231
Sequence domains: Long-chain fatty aldehyde decarbonylase
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand 6NA 1 x 6NA
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P43212
Unit cell:
a: 77.06Å b: 77.06Å c: 115.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Microbial engineering for aldehyde synthesis.
Kunjapur et al. (2015)
9 more

2 mentions without citation

Production of propane and other short-chain alkanes by structure-based engineering of ligand specificity in aldehyde-deformylating oxygenase.
Khara et al. (2013)
1 more