› 4kqj

X-ray diffraction
1.95Å resolution

Crystal structure of CobT S80Y/Q88M/L175M complexed with p-cresol and NaMN

Released:
DOI: 10.2210/pdb4kqj/pdb
PDB entry 4kqj coloured by chain, front view.
PDB entry 4kqj coloured by chain, side view.
PDB entry 4kqj coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica.
Chan CH, Newmister SA, Talyor K, Claas KR, Rayment I, Escalante-Semerena JC
Biochim Biophys Acta 1840 464-75 (2014)
PMID: 24121107

Function and Biology Details

Reaction catalysed: 
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 36.74 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05603 (Residues: 1-356; Coverage: 100%)
Gene names: STM2016, cobT
Sequence domains: Phosphoribosyltransferase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NCN 1 x NCN
Ligand PCR 1 x PCR
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 71.807Å b: 89.154Å c: 46.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.223
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus.
Suzuki et al. (2016)
11 more

1 mention without citation

Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica.
Chan et al. (2014)