› 4khb

X-ray diffraction
2.4Å resolution

Structure of the Spt16D Pob3N heterodimer

Released:
DOI: 10.2210/pdb4khb/pdb
PDB entry 4khb coloured by chain, front view.
PDB entry 4khb coloured by chain, side view.
PDB entry 4khb coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT.
Hondele M, Stuwe T, Hassler M, Halbach F, Bowman A, Zhang ET, Nijmeijer B, Kotthoff C, Rybin V, Amlacher S, Hurt E, Ladurner AG
Nature 499 111-4 (2013)
PMID: 23698368

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124083 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FACT complex subunit Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 127 amino acids
Theoretical weight: 14.72 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SDN1 (Residues: 521-647; Coverage: 12%)
Gene name: CTHT_0052370
Sequence domains: FACT complex subunit (SPT16/CDC68)
Structure domains: FACT complex subunit Spt16p/Cdc68p
FACT complex subunit POB3 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 195 amino acids
Theoretical weight: 21.83 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SHK5 (Residues: 1-192; Coverage: 34%)
Gene name: CTHT_0070340
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 85.391Å b: 128.082Å c: 132.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.244
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Histone exchange, chromatin structure and the regulation of transcription.
Venkatesh et al. (2015)
33 more

8 mentions without citation

The role of FACT in managing chromatin: disruption, assembly, or repair?
Formosa et al. (2020)
7 more