› 4keh

X-ray diffraction
1.9Å resolution

Crosslinked Crystal Structure of Type II Fatty Synthase Dehydratase, FabA, and Acyl Carrier Protein, AcpP

Released:
DOI: 10.2210/pdb4keh/pdb
PDB entry 4keh coloured by chain, front view.
PDB entry 4keh coloured by chain, side view.
PDB entry 4keh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Trapping the dynamic acyl carrier protein in fatty acid biosynthesis.
Nguyen C, Haushalter RW, Lee DJ, Markwick PR, Bruegger J, Caldara-Festin G, Finzel K, Jackson DR, Ishikawa F, O'Dowd B, McCammon JA, Opella SJ, Tsai SC, Burkart MD
Nature 505 427-31 (2014)
PMID: 24362570

Function and Biology Details

Reactions catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141416 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 18.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Q3 (Residues: 2-172; Coverage: 99%)
Gene names: JW0937, b0954, fabA
Sequence domains: FabA-like domain
Structure domains: Hotdog Thioesterase
Acyl carrier protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 77 amino acids
Theoretical weight: 8.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand 1R3 2 x 1R3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 84.405Å b: 122.178Å c: 122.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.218
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Bacterial protein networks: properties and functions.
Typas et al. (2015)
14 more

19 mentions without citation

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering.
Beld et al. (2015)
18 more