› 4jso

X-ray diffraction
2.07Å resolution

The X-ray crystal structure of a thermophilic cellobiose binding protein bound with laminaripentaose

Released:
DOI: 10.2210/pdb4jso/pdb
PDB entry 4jso coloured by chain, front view.
PDB entry 4jso coloured by chain, side view.
PDB entry 4jso coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Molecular details of ligand selectivity determinants in a promiscuous β-glucan periplasmic binding protein.
Munshi P, Stanley CB, Ghimire-Rijal S, Lu X, Myles DA, Cuneo MJ
BMC Struct Biol 13 18 (2013)
PMID: 24090243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Solute-binding protein family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 582 amino acids
Theoretical weight: 67.41 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9WXN8 (Residues: 24-605; Coverage: 100%)
Gene name: TM_0031
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 56.922Å b: 89.785Å c: 108.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.221
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

Cooperative Electrostatic Interactions Drive Functional Evolution in the Alkaline Phosphatase Superfamily.
Barrozo et al. (2015)
3 more

2 mentions without citation

A Pyranose-2-Phosphate Motif Is Responsible for Both Antibiotic Import and Quorum-Sensing Regulation in Agrobacterium tumefaciens.
El Sahili et al. (2015)
1 more