› 4js9

X-ray diffraction
2.78Å resolution

Structural Characterization of Inducible Nitric Oxide Synthase Substituted With Mesoheme

Released:
DOI: 10.2210/pdb4js9/pdb
PDB entry 4js9 coloured by chain, front view.
PDB entry 4js9 coloured by chain, side view.
PDB entry 4js9 coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Hannibal L, Page RC, Bolisetty K, Yu Z, Misra S, Stuehr DJ

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 49.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29477 (Residues: 66-496; Coverage: 38%)
Gene names: Inosl, Nos2
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand MH0 2 x MH0
2 bound ligands:
Ligand H4B 2 x H4B
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6122
Unit cell:
a: 215.039Å b: 215.039Å c: 115.547Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.25 0.29
Expression system: Escherichia coli BL21(DE3)

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