› 4jkn

X-ray diffraction
1.54Å resolution

Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A

Released:
DOI: 10.2210/pdb4jkn/pdb
PDB entry 4jkn coloured by chain, front view.
PDB entry 4jkn coloured by chain, side view.
PDB entry 4jkn coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.
Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ
J Inorg Biochem 141 152-160 (2014)
PMID: 25265377

Function and Biology Details

Reaction catalysed: 
Arsenite + H(2)O + 2 oxidized azurin = arsenate + 2 reduced azurin + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand HG 5 x HG
Ligand NO3 7 x NO3
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER
Spacegroup: P212121
Unit cell:
a: 56.483Å b: 79.228Å c: 109.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.239
Expression system: Escherichia coli

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