› 4jeq

X-ray diffraction
2.3Å resolution

Different Contribution of Conserved Amino Acids to the Global Properties of Homologous Enzymes

Released:
DOI: 10.2210/pdb4jeq/pdb
PDB entry 4jeq coloured by chain, front view.
PDB entry 4jeq coloured by chain, side view.
PDB entry 4jeq coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Different contribution of conserved amino acids to the global properties of triosephosphate isomerases.
Aguirre Y, Cabrera N, Aguirre B, Pérez-Montfort R, Hernandez-Santoyo A, Reyes-Vivas H, Enríquez-Flores S, de Gómez-Puyou MT, Gómez-Puyou A, Sanchez-Ruiz JM, Costas M
Proteins 82 323-35 (2014)
PMID: 23966267

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 250 amino acids
Theoretical weight: 26.85 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04789 (Residues: 1-250; Coverage: 100%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand PEG 5 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 88.087Å b: 89.782Å c: 181.718Å
α: 90° β: 101.61° γ: 90°
R-values:
R R work R free
0.21 0.207 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A guide to the effects of a large portion of the residues of triosephosphate isomerase on catalysis, stability, druggability, and human disease.
Olivares-Illana et al. (2017)
8 other articles

1 mention without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)