› 4j89

X-ray diffraction
2.1Å resolution

Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence

Released:
DOI: 10.2210/pdb4j89/pdb
PDB entry 4j89 coloured by chain, front view.
PDB entry 4j89 coloured by chain, side view.
PDB entry 4j89 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence.
Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD
Angew Chem Int Ed Engl 52 5974-7 (2013)
PMID: 23620472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.87 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand EDO 23 x EDO
Ligand TRS 1 x TRS
1 modified residue:
Ligand CQ2 2 x CQ2

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 52.37Å b: 98.11Å c: 102.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry.
Reddington et al. (2013)
2 more

1 mention without citation

Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein.
Olenginski et al. (2021)