› 4j7q

X-ray diffraction
1.55Å resolution

Crystal structure of Saccharomyces cerevisiae Sfh3 complexed with phosphatidylinositol

Released:
DOI: 10.2210/pdb4j7q/pdb
PDB entry 4j7q coloured by chain, front view.
PDB entry 4j7q coloured by chain, side view.
PDB entry 4j7q coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural determinants for phosphatidylinositol recognition by Sfh3 and substrate-induced dimer-monomer transition during lipid transfer cycles.
Yang H, Tong J, Leonard TA, Im YJ
FEBS Lett 587 1610-6 (2013)
PMID: 23603387

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-156894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol transfer protein PDR16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 38.6 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53860 (Residues: 15-345; Coverage: 94%)
Gene names: N1158, PDR16, SFH3, YNL231C
Sequence domains:
Structure domains: CRAL-TRIO lipid binding domain

Ligands and Environments

1 bound ligand:
Ligand B7N 2 x B7N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 52.889Å b: 144.149Å c: 55.656Å
α: 90° β: 111.28° γ: 90°
R-values:
R R work R free
0.222 0.221 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Lipid Exchangers: Cellular Functions and Mechanistic Links With Phosphoinositide Metabolism.
Lipp et al. (2020)
4 other articles

3 mentions without citation

Interaction of Fapp1 with Arf1 and PI4P at a membrane surface: an example of coincidence detection.
Liu et al. (2014)
2 more