› 4j7g

X-ray diffraction
1.7Å resolution

Crystal structure of EvaA, a 2,3-dehydratase in complex with dTDP-fucose and dTDP-rhamnose

Released:
DOI: 10.2210/pdb4j7g/pdb
PDB entry 4j7g coloured by chain, front view.
PDB entry 4j7g coloured by chain, side view.
PDB entry 4j7g coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Primary publication:
Structure of EvaA: a paradigm for sugar 2,3-dehydratases.
Kubiak RL, Thoden JB, Holden HM
Biochemistry 52 2078-88 (2013)
PMID: 23473392

Function and Biology Details

Reaction catalysed: 
(1a) dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose = dTDP-2,6-dideoxy-D-glycero-hex-2-enos-4-ulose + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-129413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 471 amino acids
Theoretical weight: 53 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52793 (Residues: 1-471; Coverage: 100%)
Gene names: Orf23, evaA
Sequence domains: NDP-hexose 2,3-dehydratase
Structure domains: EvaA sugar 2,3-dehydratase subunit

Ligands and Environments

2 bound ligands:
Ligand TRH 2 x TRH
Ligand 1JB 2 x 1JB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 105.829Å b: 108.218Å c: 110.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Nucleotide sugar dehydratases: Structure, mechanism, substrate specificity, and application potential.
Vogel et al. (2022)
1 other article