› 4j5k

X-ray diffraction
1.23Å resolution

Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa Y51F mutant

Released:
DOI: 10.2210/pdb4j5k/pdb
PDB entry 4j5k coloured by chain, front view.
PDB entry 4j5k coloured by chain, side view.
PDB entry 4j5k coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Primary publication:
Contribution of hydrogen bonds to protein stability.
Pace CN, Fu H, Lee Fryar K, Landua J, Trevino SR, Schell D, Thurlkill RL, Imura S, Scholtz JM, Gajiwala K, Sevcik J, Urbanikova L, Myers JK, Takano K, Hebert EJ, Shirley BA, Grimsley GR
Protein Sci 23 652-61 (2014)
PMID: 24591301

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-138777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05798 (Residues: 1-96; Coverage: 100%)
Gene name: rnaSA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 38.303Å b: 63.813Å c: 77.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.106 0.104 0.136
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Forces stabilizing proteins.
Nick Pace et al. (2014)
2 more

2 mentions without citation

Contribution of hydrogen bonds to protein stability.
Pace et al. (2014)
1 more