› 4j4p

X-ray diffraction
2.91Å resolution

The complex of human IgE-Fc with two bound Fab fragments

Released:
DOI: 10.2210/pdb4j4p/pdb
PDB entry 4j4p coloured by chain, front view.
PDB entry 4j4p coloured by chain, side view.
PDB entry 4j4p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human immunoglobulin E flexes between acutely bent and extended conformations.
Drinkwater N, Cossins B, Keeble AH, Wright M, Cain K, Hailu H, Oxbrow A, Delgado J, Shuttleworth LK, Kao MW, McDonnell JM, Beavil AJ, Henry AJ, Sutton BJ
Nat Struct Mol Biol 21 397-404 (2014)
PMID: 24632569

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208609 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 35.93 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P01854 (Residues: 105-427; Coverage: 59%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin G Fab Fragment Heavy Chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 249 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Immunoglobulin G Fab Fragment Light Chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 235 amino acids
Theoretical weight: 24.84 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 84.59Å b: 100.81Å c: 219.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.284
Expression systems:
  • Mus musculus
  • Cricetulus griseus

Quick links

Citations

11 review citations

Structure and dynamics of IgE-receptor interactions: FcεRI and CD23/FcεRII.
Sutton et al. (2015)
10 more

8 mentions without citation

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions.
Lefranc et al. (2020)
7 more