› 4j4j

X-ray diffraction
3.1Å resolution

Crystal structure of the APOBEC3F Vif binding domain

Released:
DOI: 10.2210/pdb4j4j/pdb
PDB entry 4j4j coloured by chain, front view.
PDB entry 4j4j coloured by chain, side view.
PDB entry 4j4j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F.
Siu KK, Sultana A, Azimi FC, Lee JE
Nat Commun 4 2593 (2013)
PMID: 24185281

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3F Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 24.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IUX4 (Residues: 218-373; Coverage: 42%)
Gene name: APOBEC3F
Sequence domains: APOBEC-like N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I422
Unit cell:
a: 164.046Å b: 164.046Å c: 135.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.236 0.277
Expression system: Escherichia coli

Quick links

Citations

13 review citations

APOBECs and virus restriction.
Harris et al. (2015)
12 more

13 mentions without citation

The APOBEC Protein Family: United by Structure, Divergent in Function.
Salter et al. (2016)
12 more