› 4ixn

X-ray diffraction
2.05Å resolution

Crystal Structure of Zn(II)-bound E37A,C66A,C67A triple mutant YjiA GTPase

Released:
DOI: 10.2210/pdb4ixn/pdb
PDB entry 4ixn coloured by chain, front view.
PDB entry 4ixn coloured by chain, side view.
PDB entry 4ixn coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Metal binding properties of Escherichia coli YjiA, a member of the metal homeostasis-associated COG0523 family of GTPases.
Sydor AM, Jost M, Ryan KS, Turo KE, Douglas CD, Drennan CL, Zamble DB
Biochemistry 52 1788-1801 (2013)
PMID: 24449932

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc chaperone YjiA Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 35.57 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24203 (Residues: 1-318; Coverage: 100%)
Gene names: JW5790, b4352, yjiA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 56.071Å b: 68.436Å c: 77.699Å
α: 90° β: 104.23° γ: 90°
R-values:
R R work R free
0.204 0.203 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Recent developments in copper and zinc homeostasis in bacterial pathogens.
Braymer et al. (2014)
7 more

1 mention without citation

Metal binding properties of Escherichia coli YjiA, a member of the metal homeostasis-associated COG0523 family of GTPases.
Sydor et al. (2013)