› 4ie6

X-ray diffraction
2.5Å resolution

Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with N-[(1-chloro-4-hydroxyisoquinolin-3-yl)carbonyl]glycine (IOX3/UN9)

Released:
DOI: 10.2210/pdb4ie6/pdb
PDB entry 4ie6 coloured by chain, front view.
PDB entry 4ie6 coloured by chain, side view.
PDB entry 4ie6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for inhibition of the fat mass and obesity associated protein (FTO).
Aik W, Demetriades M, Hamdan MK, Bagg EA, Yeoh KK, Lejeune C, Zhang Z, McDonough MA, Schofield CJ
J Med Chem 56 3680-8 (2013)
PMID: 23547775

Function and Biology Details

Reaction catalysed: 
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent dioxygenase FTO Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 56.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C0B1 (Residues: 32-505; Coverage: 94%)
Gene names: FTO, KIAA1752
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand UN9 1 x UN9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 141.458Å b: 141.458Å c: 83.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.21
Expression system: Escherichia coli

Quick links

Citations

30 review citations

N6-methyladenosine links RNA metabolism to cancer progression.
Dai et al. (2018)
29 more

4 mentions without citation

Structural Insights into N6-methyladenosine (m6A) Modification in the Transcriptome.
Huang et al. (2018)
3 more