› 4idp

X-ray diffraction
2.59Å resolution

human atlastin-1 1-446, N440T, GppNHp

Released:
DOI: 10.2210/pdb4idp/pdb
PDB entry 4idp coloured by chain, front view.
PDB entry 4idp coloured by chain, side view.
PDB entry 4idp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for conformational switching and GTP loading of the large G protein atlastin.
Byrnes LJ, Singh A, Szeto K, Benvin NM, O'Donnell JP, Zipfel WR, Sondermann H
EMBO J 32 369-84 (2013)
PMID: 23334294

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Atlastin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 51.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WXF7 (Residues: 1-446; Coverage: 80%)
Gene names: ATL1, GBP3, SPG3A
Sequence domains: Guanylate-binding protein, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GNP 4 x GNP
Ligand MG 4 x MG
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 131.946Å b: 268.121Å c: 62.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.236
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Form follows function: the importance of endoplasmic reticulum shape.
Westrate et al. (2015)
12 more

3 mentions without citation

Invited review: Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily.
Daumke et al. (2016)
2 more