› 4ich

X-ray diffraction
1.95Å resolution

Crystal structure of a putative TetR family transcriptional regulator from Saccharomonospora viridis DSM 43017

Released:
DOI: 10.2210/pdb4ich/pdb
PDB entry 4ich coloured by chain, front view.
PDB entry 4ich coloured by chain, side view.
PDB entry 4ich coloured by chain, top view.
Source organism: Saccharomonospora viridis DSM 43017
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Jedrzejczak R, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 35.75 KDa
Source organism: Saccharomonospora viridis DSM 43017
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7MT25 (Residues: 1-287; Coverage: 100%)
Gene name: Svir_16270
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

4 bound ligands:
Ligand BME 2 x BME
Ligand B3P 2 x B3P
Ligand BR 1 x BR
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 42.845Å b: 76.929Å c: 109.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.247
Expression system: Escherichia coli BL21(DE3)

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