› 4i8l

X-ray diffraction
0.87Å resolution

Bovine trypsin at 0.87 resolution

Released:
DOI: 10.2210/pdb4i8l/pdb
PDB entry 4i8l coloured by chain, front view.
PDB entry 4i8l coloured by chain, side view.
PDB entry 4i8l coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
On the reproducibility of protein crystal structures: five atomic resolution structures of trypsin.
Liebschner D, Dauter M, Brzuszkiewicz A, Dauter Z
Acta Crystallogr D Biol Crystallogr 69 1447-62 (2013)
PMID: 23897468

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand BEN 1 x BEN
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 54.219Å b: 58.345Å c: 66.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.103 0.102 0.117

Quick links

Citations

1 review citation

The Molecular Origin of Enthalpy/Entropy Compensation in Biomolecular Recognition.
Fox et al. (2018)
25 other articles

3 mentions without citation

On the reproducibility of protein crystal structures: five atomic resolution structures of trypsin.
Liebschner et al. (2013)
2 more