› 4i5k

X-ray diffraction
2.9Å resolution

PP2A PR70 Holoenzyme model3_diCa_rcsb.pdb bppnat5_extend.mtz

Released:
DOI: 10.2210/pdb4i5k/pdb
PDB entry 4i5k coloured by chain, front view.
PDB entry 4i5k coloured by chain, side view.
PDB entry 4i5k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Ca2+-dependent PP2A heterotrimer and insights into Cdc6 dephosphorylation.
Wlodarchak N, Guo F, Satyshur KA, Jiang L, Jeffrey PD, Sun T, Stanevich V, Mumby MC, Xing Y
Cell Res 23 931-46 (2013)
PMID: 23752926

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase 2A regulatory subunit B" subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 33.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06190 (Residues: 786-1070; Coverage: 25%)
Gene names: PPP2R3, PPP2R3A
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 132.812Å b: 109.086Å c: 67.651Å
α: 90° β: 109.05° γ: 90°
R-values:
R R work R free
0.21 0.207 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

All roads lead to PP2A: exploiting the therapeutic potential of this phosphatase.
Sangodkar et al. (2016)
14 more

1 mention without citation

Structure of the Ca2+-dependent PP2A heterotrimer and insights into Cdc6 dephosphorylation.
Wlodarchak et al. (2013)