› 4hv6

X-ray diffraction
2.3Å resolution

Structure of MNTR H77A mutant in apo- and mn-bound forms

Released:
DOI: 10.2210/pdb4hv6/pdb
PDB entry 4hv6 coloured by chain, front view.
PDB entry 4hv6 coloured by chain, side view.
PDB entry 4hv6 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Roles of the A and C sites in the manganese-specific activation of MntR.
McGuire AM, Cuthbert BJ, Ma Z, Grauer-Gray KD, Brunjes Brophy M, Spear KA, Soonsanga S, Kliegman JI, Griner SL, Helmann JD, Glasfeld A
Biochemistry 52 701-13 (2013)
PMID: 23298157

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator MntR Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 16.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P54512 (Residues: 2-142; Coverage: 99%)
Gene names: BSU24520, mntR, yqhN
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 51.734Å b: 51.734Å c: 175.564Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.252 0.317
Expression system: Escherichia coli

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Citations

12 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
11 more

1 mention without citation

Roles of the A and C sites in the manganese-specific activation of MntR.
McGuire et al. (2013)