› 4hmv

X-ray diffraction
1.45Å resolution

Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with tetrahydrophenazine-1-carboxylic acid after 5 days of soaking

Released:
DOI: 10.2210/pdb4hmv/pdb
PDB entry 4hmv coloured by chain, front view.
PDB entry 4hmv coloured by chain, side view.
PDB entry 4hmv coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
Acta Crystallogr D Biol Crystallogr 69 1403-13 (2013)
PMID: 23897464

Function and Biology Details

Reaction catalysed: 
(1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate + O(2) = (10aS)-5,10-dihydrophenazine-1-carboxylate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrophenazinedicarboxylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.25 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51793 (Residues: 1-222; Coverage: 100%)
Gene name: phzG
Sequence domains:
Structure domains: Electron Transport, Fmn-binding Protein; Chain A

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand SO4 1 x SO4
Ligand WUB 1 x WUB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 57.668Å b: 63.575Å c: 133.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.15
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Phenazines in plant-beneficial Pseudomonas spp.: biosynthesis, regulation, function and genomics.
Biessy et al. (2018)
2 more

2 mentions without citation

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa.
Froes et al. (2019)
1 more