› 4hhg

X-ray diffraction
1.6Å resolution

Crystal structure of the Pseudomonas aeruginosa azurin, RuH107NO YOH109

Released:
DOI: 10.2210/pdb4hhg/pdb
PDB entry 4hhg coloured by chain, front view.
PDB entry 4hhg coloured by chain, side view.
PDB entry 4hhg coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Electron flow through nitrotyrosinate in Pseudomonas aeruginosa azurin.
Warren JJ, Herrera N, Hill MG, Winkler JR, Gray HB
J Am Chem Soc 135 11151-8 (2013)
PMID: 23859602

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 14.05 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand DRU 1 x DRU
1 modified residue:
Ligand NIY 1 x NIY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I222
Unit cell:
a: 49.762Å b: 67.176Å c: 81.385Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.261
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Electron flow through metalloproteins.
Winkler et al. (2014)
4 more

1 mention without citation

Electron flow through nitrotyrosinate in Pseudomonas aeruginosa azurin.
Warren et al. (2013)