4h5l

X-ray diffraction
2.75Å resolution

Crystal Structure of Toscana Virus Nucleocapsid Protein Hexamer

Released:
Source organism: Toscana virus
Primary publication:
Phleboviruses encapsidate their genomes by sequestering RNA bases.
Proc Natl Acad Sci U S A 109 19208-13 (2012)
PMID: 23129612

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 27.74 KDa
Source organism: Toscana virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21701 (Residues: 1-253; Coverage: 100%)
Gene name: N
Sequence domains: Tenuivirus/Phlebovirus nucleocapsid protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 50.35Å b: 93.75Å c: 95.52Å
α: 67.92° β: 85.94° γ: 87.95°
R-values:
R R work R free
0.22 0.219 0.238
Expression system: Escherichia coli BL21