› 4h47

X-ray diffraction
1.9Å resolution

1.9 angstrom CyPet structure at pH5.2

Released:
DOI: 10.2210/pdb4h47/pdb
PDB entry 4h47 coloured by chain, front view.
PDB entry 4h47 coloured by chain, side view.
PDB entry 4h47 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Hu X-J, Liu R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 62.64Å c: 70.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.243
Expression system: Escherichia coli

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