› 4h1v

X-ray diffraction
2.3Å resolution

GMP-PNP bound dynamin-1-like protein GTPase-GED fusion

Released:
DOI: 10.2210/pdb4h1v/pdb
PDB entry 4h1v coloured by chain, front view.
PDB entry 4h1v coloured by chain, side view.
PDB entry 4h1v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional mapping of human dynamin-1-like GTPase domain based on x-ray structure analyses.
Wenger J, Klinglmayr E, Fröhlich C, Eibl C, Gimeno A, Hessenberger M, Puehringer S, Daumke O, Goettig P
PLoS One 8 e71835 (2013)
PMID: 23977156

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynamin-1-like protein Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 40.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00429 (Residues: 1-327, 339-736; Coverage: 48%)
Gene names: DLP1, DNM1L, DRP1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 53.42Å b: 151.31Å c: 43.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.278
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Membrane fission by dynamin: what we know and what we need to know.
Antonny et al. (2016)
16 more

5 mentions without citation

Functional mapping of human dynamin-1-like GTPase domain based on x-ray structure analyses.
Wenger et al. (2013)
4 more