› 4gx3

X-ray diffraction
2.25Å resolution

Product Complexes of Porcine Liver Fructose-1,6-bisphosphatase with mutation R22M Reveal a T-state Conformation

Released:
DOI: 10.2210/pdb4gx3/pdb
PDB entry 4gx3 coloured by chain, front view.
PDB entry 4gx3 coloured by chain, side view.
PDB entry 4gx3 coloured by chain, top view.
Source organism: Sus scrofa
Entry authors: Gao Y, Honzatko RB

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 36.67 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00636 (Residues: 2-338; Coverage: 100%)
Gene names: FBP, FBP1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand F6P 2 x F6P
Ligand MG 4 x MG
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 59.249Å b: 165.012Å c: 79.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.27
Expression system: Escherichia coli

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