› 4gwn

X-ray diffraction
3Å resolution

Crystal structure of human mature meprin beta

Released:
DOI: 10.2210/pdb4gwn/pdb
PDB entry 4gwn coloured by chain, front view.
PDB entry 4gwn coloured by chain, side view.
PDB entry 4gwn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the sheddase function of human meprin β metalloproteinase at the plasma membrane.
Arolas JL, Broder C, Jefferson T, Guevara T, Sterchi EE, Bode W, Stöcker W, Becker-Pauly C, Gomis-Rüth FX
Proc Natl Acad Sci U S A 109 16131-6 (2012)
PMID: 22988105

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His(5)-|-Leu-, -Leu(6)-|-Cys-, -Ala(14)-|-Leu- and -Cys(19)-|-Gly- bonds in insulin B chain.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Meprin A subunit beta Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 62.81 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q16820 (Residues: 62-614; Coverage: 81%)
Gene name: MEP1B
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand CD 1 x CD
Ligand NA 1 x NA
Ligand NAG 2 x NAG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 74.96Å b: 74.96Å c: 502.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.238
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Proteolytic ectodomain shedding of membrane proteins in mammals-hardware, concepts, and recent developments.
Lichtenthaler et al. (2018)
7 more

12 mentions without citation

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
11 more