› 4guv

X-ray diffraction
2.73Å resolution

TetX derivatized with Xenon

Released:
DOI: 10.2210/pdb4guv/pdb
PDB entry 4guv coloured by chain, front view.
PDB entry 4guv coloured by chain, side view.
PDB entry 4guv coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
Putative dioxygen-binding sites and recognition of tigecycline and minocycline in the tetracycline-degrading monooxygenase TetX.
Volkers G, Damas JM, Palm GJ, Panjikar S, Soares CM, Hinrichs W
Acta Crystallogr D Biol Crystallogr 69 1758-67 (2013)
PMID: 23999299

Function and Biology Details

Reaction catalysed: 
Tetracycline + NADPH + O(2) = 11a-hydroxytetracycline + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent monooxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 44.7 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93L51 (Residues: 11-388; Coverage: 97%)
Gene name: tetX2
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand SO4 11 x SO4
Ligand XE 8 x XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P1
Unit cell:
a: 68.71Å b: 80.14Å c: 87.5Å
α: 111.04° β: 90.06° γ: 93.29°
R-values:
R R work R free
0.183 0.181 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Tetracycline-Inactivating Enzymes.
Markley et al. (2018)
2 more