› 4guk

X-ray diffraction
1.75Å resolution

New crystal form structure of human NCS1

Released:
DOI: 10.2210/pdb4guk/pdb
PDB entry 4guk coloured by chain, front view.
PDB entry 4guk coloured by chain, side view.
PDB entry 4guk coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fan C, Lolis E

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal calcium sensor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62166 (Residues: 6-188; Coverage: 96%)
Gene names: FLUP, FREQ, NCS1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

7 bound ligands:
Ligand PG4 7 x PG4
Ligand EDO 5 x EDO
Ligand CA 12 x CA
Ligand NA 4 x NA
Ligand P3G 1 x P3G
Ligand P2G 4 x P2G
Ligand ALA 1 x ALA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P31
Unit cell:
a: 54.987Å b: 54.987Å c: 213.954Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.243
Expression system: Escherichia coli BL21(DE3)

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