› 4gsj

X-ray diffraction
1.7Å resolution

Crystal structure of Atg7 NTD K14A F16A D18A mutant

Released:
DOI: 10.2210/pdb4gsj/pdb
PDB entry 4gsj coloured by chain, front view.
PDB entry 4gsj coloured by chain, side view.
PDB entry 4gsj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.
Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA
Nat Struct Mol Biol 19 1242-9 (2012)
PMID: 23142976

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like modifier-activating enzyme ATG7 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38862 (Residues: 1-289; Coverage: 46%)
Gene names: APG7, ATG7, CVT2, YHR171W
Sequence domains: Ubiquitin-like modifier-activating enzyme ATG7 N-terminus

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand IPA 6 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 56.826Å b: 74.631Å c: 76.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

The machinery of macroautophagy.
Feng et al. (2014)
23 more

1 mention without citation

Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.
Kaiser et al. (2012)