› 4goc

X-ray diffraction
2.4Å resolution

Crystal structure of the Get5 ubiquitin-like domain

Released:
DOI: 10.2210/pdb4goc/pdb
PDB entry 4goc coloured by chain, front view.
PDB entry 4goc coloured by chain, side view.
PDB entry 4goc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
Chartron JW, VanderVelde DG, Clemons WM
Cell Rep 2 1620-32 (2012)
PMID: 23142665

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like protein MDY2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 76 amino acids
Theoretical weight: 8.54 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12285 (Residues: 74-148; Coverage: 35%)
Gene names: GET5, MDY2, TMA24, YOL111C
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 49.36Å b: 63.62Å c: 71.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.174 0.245
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.
Chio et al. (2017)
10 more

3 mentions without citation

Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
Chartron et al. (2012)
2 more