› 4gml

X-ray diffraction
2.9Å resolution

Crystal structure of human NOT1 MIF4G domain

Released:
DOI: 10.2210/pdb4gml/pdb
PDB entry 4gml coloured by chain, front view.
PDB entry 4gml coloured by chain, side view.
PDB entry 4gml coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O
Nucleic Acids Res 40 11058-72 (2012)
PMID: 22977175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCR4-NOT transcription complex subunit 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 235 amino acids
Theoretical weight: 27.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A5YKK6 (Residues: 1093-1317; Coverage: 10%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 88.27Å b: 133.823Å c: 331.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.229
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Towards a molecular understanding of microRNA-mediated gene silencing.
Jonas et al. (2015)
17 more

2 mentions without citation

The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
Petit et al. (2012)
1 more